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Bosses in Pikmin series are larger and stronger than other enemies, and may require some specific task to take down. Often, bosses have attacks capable of killing many Pikmin at once. For this reason, a tight-knit group is generally more effective against bosses than a large army.

When fighting bosses, a very intense piece of music plays to make the battle more engaging. The boss music is very varied across Pikmin bosses, Pikmin 2 bosses, and Pikmin 3 bosses.